2x sybr green stain solution Search Results


2x sybr gold  (Thermo Fisher)
99
Thermo Fisher 2x sybr gold
2x Sybr Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext high fidelity 2x pcr master mix neb  (New England Biolabs)
97
New England Biolabs nebnext high fidelity 2x pcr master mix neb
Nebnext High Fidelity 2x Pcr Master Mix Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2x sybr green stain solution  (Thermo Fisher)
90
Thermo Fisher 2x sybr green stain solution
2x Sybr Green Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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2x sybr green i nucleic acid gel stain  (Thermo Fisher)
86
Thermo Fisher 2x sybr green i nucleic acid gel stain
2x Sybr Green I Nucleic Acid Gel Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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2x sybr ® green  (Lonza)
90
Lonza 2x sybr ® green
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
2x Sybr ® Green, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95047 500 qpcr bio sygreen mix lo rox pcr biosystems pb20 11 05 dream taq green pcr master mix 2x  (PCR Biosystems Ltd)
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PCR Biosystems Ltd 95047 500 qpcr bio sygreen mix lo rox pcr biosystems pb20 11 05 dream taq green pcr master mix 2x
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
95047 500 Qpcr Bio Sygreen Mix Lo Rox Pcr Biosystems Pb20 11 05 Dream Taq Green Pcr Master Mix 2x, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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2x sybr green i dna dye  (Thermo Fisher)
99
Thermo Fisher 2x sybr green i dna dye
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
2x Sybr Green I Dna Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2x chamq sybr qpcr master mix vazyme cat  (Vazyme Biotech Co)
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Vazyme Biotech Co 2x chamq sybr qpcr master mix vazyme cat
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
2x Chamq Sybr Qpcr Master Mix Vazyme Cat, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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2x laemmli sample buffer bio rad  (Bio-Rad)
97
Bio-Rad 2x laemmli sample buffer bio rad
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
2x Laemmli Sample Buffer Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2x sybr safe stain  (Thermo Fisher)
90
Thermo Fisher 2x sybr safe stain
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
2x Sybr Safe Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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am9759 nebnext high fidelity 2x pcr master mix new england biolabs  (New England Biolabs)
97
New England Biolabs am9759 nebnext high fidelity 2x pcr master mix new england biolabs
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
Am9759 Nebnext High Fidelity 2x Pcr Master Mix New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 x sybr green i nucleic acid gel stain  (Thermo Fisher)
90
Thermo Fisher 2 x sybr green i nucleic acid gel stain
(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using <t>SYBR</t> <t>Green</t> <t>I</t> staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.
2 X Sybr Green I Nucleic Acid Gel Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


(A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using SYBR Green I staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.

Journal: bioRxiv

Article Title: Quinazoline-quinoline bisubstrate inhibitors target eukaryotic translation initiation factor 3 in Plasmodium falciparum

doi: 10.1101/2022.12.10.519887

Figure Lengend Snippet: (A) Strategy to generate PfEIF3i-HARibo parasite line with 3xHA tag followed by a glmS ribozyme sequence. HR, homology region; arrow boxes, coding sequences; arrows, 5′UTR; open circle lollipop, 3’UTR; black box hDHFR, selection marker cassette; red box, 3xHA-tag; green box, glmS ribozyme cassette; yellow box, T2A peptide; grey box, yDHOH cassette; grey arrows P1 and P2, primers used for the PCR. (B) After transfection, the integration was validated by PCR and correct HA expression was confirmed by western blot using anti-HA antibodies. (C) Extracts of PfEIF3i-HARibo clones C7 and F12 were collected after saponin lysis from parasite cultured with or without glucosamine (GlcN) for one cycle. EIF3i protein levels were quantified by western blot using anti-HA monoclonal antibodies and anti-H3 antibodies as loading controls. (D) Parasite were cultivated in presence or not of GlcN (added at day 0, D0) and growth was assessed for 4 days, every 24h, by flow cytometry using SYBR Green I staining. (E) Synchronized parasites were cultivated in presence or not of GlcN (added at T0h) and blood smears were realized regularly at ring, trophozoites and schizont stages.

Article Snippet: Fixed cells were then stained with 2X SYBR ® Green I (Lonza, 50512) and parasitemia was determined by flow cytometry using the Guava ® EasyCyte™ HT flow cytometer (Luminex).

Techniques: Sequencing, Selection, Marker, Transfection, Expressing, Western Blot, Clone Assay, Lysis, Cell Culture, Flow Cytometry, SYBR Green Assay, Staining